2021.01.23 11:56 DonPecz Ghetto wall in Warsaw in 1943 vs present day.
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2021.01.23 11:56 Bunnyjets My sweet pre-demon baby girl ❤️
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2021.01.23 11:56 1913intel Brown Ground Meat Faster (and Keep It More Moist) With Baking Soda
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2021.01.23 11:56 str8red Update on the Americas
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2021.01.23 11:56 axw420 Warhead
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2021.01.23 11:56 Zabalacuochi Earth go brrrr
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2021.01.23 11:56 obries39 How to subtract SERS background?
Hey all, I'd really appreciate some advice with a problem I'm currently having in the lab.
So I'm carrying out surface enhanced Raman spectroscopy (SERS) measurements on human cerebrospinal fluid (CSF).
I detected a strong signal from bovine serum albumin (BSA) in micromolar concentrations which I used as a calibration standard so as not to waste the human samples. However, when I measured the actual CSF I couldn't detect anything other than the peaks from the substrate itself (Ocean Optics Au Nanoparticle substrate for 785 nm).
I presume the concentration of the actual CSF was far lower than anticipated (unfortunately we don't know the concentration) and our volume of sample is incredibly small (10 uL max). Unfortunately our Raman system is not confocal and not an imaging system. So I can't see precisely where I'm measuring.
However, some interest things I noted in the data, the signal from the substrate alone is far higher than the actual signal, I presume this is due to the dried CSF shielding the Au NP surface. But that means a simple reference subtraction isn't possible.
I do think there is some signal burried under the background signal because for some sample I was able to remove the baseline using asymmetric least squares smoothing, normalising, subtracting the reference, and then taking the second derivate. When I did this I notice a low signal where the Amide III and Phenylalanine peaks should be, but only for some of the data.
So my question is two fold:
1) Is there a better way that I can subtract the reference background to reveal the signal?
2) Is there anything I can do to enhance the signal when measuring in the future? (Unfortunately increasing the concentration isn't possible).
Thanks in advance!
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2021.01.23 11:56 Dylangraphic23 Would You Rather Have Sex with Women or Play Violent Video Games
2021.01.23 11:56 androidblack EarthQuake PRO Apk 15.1.3-PRO [Paid] for Android
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2021.01.23 11:56 stealthswor NIMBY Harrow Council reject housing plans in order to preserve car park
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2021.01.23 11:56 ulenie1 Another cat theft victim
2021.01.23 11:56 -stan-kpop Chaeyoung
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2021.01.23 11:56 AquilesCuentoo So basically the last 3 DLCs were leaked by the early art book that came out in 2018
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2021.01.23 11:56 My_Memes_Will_Cure_U Edward Mordrake, the man born with a second face on back of his head
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2021.01.23 11:56 Shitazzboi Why can’t my shitty laptop run realm Royale on steam or discord I just brought nitro and I can’t get the nitro rewards
2021.01.23 11:56 longhegrindilemna Favoritism
2021.01.23 11:56 notrick22 COR sucht Support platin/dia
wir sind Carnage on the Rift und suchen aktuell nach:
- einem Support
Was ihr mitbringen solltet: - Platin/Diamond Elo
- angemessener Champpool
- wöchentlich mind. Zeit für 2 Trainings- und einen Spieltag (ab 19 Uhr unter der Woche)
- Aktivität (Solo/Flexq)
- Wille sich zu verbessern
Zu uns: - nicht organisationsgebunden
- Erfahrung im Teamplay - Aufgabenverteilung untereinander, kein „Captain“
- 2 Platin, 2 Diamond Spieler
- Teilnahme an der Primeleague und evtl. andere Turniere
Bei Interesse bitte eine Nachricht an mich mit einem op.gg link
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2021.01.23 11:56 Impossible_News_3765 I ship yami x momo
2021.01.23 11:56 markANTHONYgb Self aware toxic people of Reddit, what made you realise you were toxic and what steps have you taken to improve?
2021.01.23 11:56 Puzzleheaded_Boss728 Lessons learned
So I'm a high end player. Im heading back to the coast like I do on occasion to help out fresh spawn and look for some sewing kits etc.
I'm well kitted out with my M4 and bullet proof vest. I spot a low end player scoping me in the distance.. I'll head over and say hi I thought. I'm generally not a kos player and his blue backpack and orange jumpsuit scream friendly. I sprint toward him and our pops an SG5K and he sprays me until I'm unconscious..zombies finish me off while I'm regaining consciousness.
Bye bye M4, NVGs, M70, hunting gear, cooking pot and all my other gear..
Maybe being friendly doesn't pay...
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2021.01.23 11:56 allheiltoaster Spooky ghost done, C&C welcome
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2021.01.23 11:56 kangaburra this is my first fan art
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2021.01.23 11:56 r_milpool_nixon Nixon Heeds Call of Masses to Return to Politics in the House, Calls Dems ‘Gay’
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2021.01.23 11:56 MartyChavez01 Neon Genesis
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2021.01.23 11:56 fart4206911 Favourite film to feature an AC/DC song.
ACDC is notorious when it comes to songs featuring in movies such as Battleship, ironman 1 and 2 and even films like grown ups and the list goes on, I wanted to know which film to feature an ACDC song is your favourite.
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